Rapid detection of listeria monocytogenes in food with multiplex - nested pcr 检测食品中单增李斯特菌研究
Study of the nested pcr detection salmonella typhi in the blood specimens 巢式聚合酶链反应快速检测血液标本中
Definition of nested pcr 巢式pcr的定义
By using the method of genome walking , two fragments were cloned from genome walker library after the semi - nested pcr . the result of sequencing the bigger fragment showed that this 706bp sequence contained the atg start codon 通过半巢式的基因步移法,经二轮pcr后,从genomewalker文库分离到2个片段,较大的片段测序表明该706bp序列含有编码ast的起始密码子atg ,其上游序列为启动子序列。
nested pcr meaning:[Medicine] In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The ess...